Parasitic infection is a serious health problem especially in developing countries. In the present study, a total of 700 school children of different sex and age from Beni Suief Governorate were examined. Urine and stool samples were collected from each child for parasitological examination by direct smear and concentration techniques (simple sedimentation method, formalin ether sedimentation and zinc sulfate floatation techniques. Perianal swab was performed among children complaining of perianal itching. Also, stool samples were stained by modified Ziehl Neelsen stain. The commonest helminthic infections were H. nana followed by E. vermicularis, A. lumbricoides and then S.haematobium, while the commonest protozoal infections were E. histolytica followed by G. lamblia and then E.coli. All stool samples examined were negative for C. parvum.Parasitic infections were more prevalent among primary school children than preparatory school children and among males than females. Social standards were shown to have significant effect on prevalence of parasitic infections, which more prevalent in very low and low classes than middle class. Parasitic infections were higher in rural areas than semiurban areas.Stool samples were examined by different methods, which showed that formalin ether sedimentation and zinc sulfate floatation techniques were more accurate than simple sedimentation method which was more accurate than direct smear. Also, perianal swab was more accurate way for detection of E. vermicularis as it detected more cases than direct smear and concentration techniques. the most common symptoms associated with parasitic infections were abdominal colic followed by diarrhea, nausea and vomiting, passage of worm, tenesmus, constipation and then loss of weight.