Objective : to assess hearing of thalassemia patients and to study effect of DFO on hearing of these patients. Background: Thalassemia major is a severe anemia of childhood, with the improved life expectations of beta thalassemia major a clinical problem such as hearing damage must be evaluated. Methodology: all cases were subjected to careful history taking, physical examination & audiological examination in the form of conventional PTA threshold detection, extended high frequency threshold detection, speech audiometric tests, Immittancemetry tests, ABR, TEOAE’s. Material: fifteen normal volunteers (control group) and 29 thalassemia major patients (study group) the age range of study group was 6-22 years old .Their mean age was 15.3 4.6. Results: 48.3% of patients had SNHL mainly high frequency SNHL at 8 to 18 KHz, 51.7% of patients had normal hearing threshold. The age, serum ferritin level, DFO dose & duration, serum Hb level did not show a significant difference between patients with & without hearing loss. Conclusion : Beta thalassemia patients are susceptible to SNHL, hearing loss usually affect high frequency, no correlation between hearing level & age, rate of blood transfusion, DFO duration & DFO dose.