We sought to evaluate the efficacy of enzymatic digestion (ED) of testicular tissue harvested from patients with non obstructive azoospermia (NOA) over the treatment of testicular tissue by mincing and Erythrocyte Lysing Buffer (ELB). Ninety one patients underwent testicular sperm extraction procedure. Fifty patients underwent diagnostic TESE (Group A) .On the other hand, the remaining 41 patients who underwent therapeutic TESE/ICSI and multiple samples were taken from each testis (Group B). In group A each sample was divided into aproximately two equal weighed fractions. The first fraction was treated by mincing and ELB and the second one was subjected to mincing and ED protocol. Each sample in group B was subjected to FE, ELB, and treatment of the remaining testicular tissue pellet with ED. Testicular tissue processing with ELB, and ED of testicular tissue increased the sperm recovery rate from (19.9%) to (26.4%) however, the increments in both groups were not significant. On the other hand, there were insignificant increase in sperm count, motility and motile sperm count after processing of testicular tissue with collagenase IV and DNase. Treatment of testicular tissue with collagenase and DNase costs 5 to 60 US$ . If we add the time factor into consideration we will find that the procedure itself may take 90 to 120 minutes. According to our results, enzymatic dissolution of testicular tissue for patients with (NOA), is neither cheap nor is an efficient way to increase sperm retrieval rate in such patients.