The tegumental antigen prepared by Triton X 100 for using ELISA and SDS-PAGE and EITB. Sera samples were collected from 5 groups : mansoni infected patients, fasciola infected patients, other parasites infected patient and normal group.The result of ELISA has shown cross reactivity between cases of fasciola and S. mansoni tegument antigen and no reaction recorded between hydatid or ascaris with S. mansoni and with EITB there is two bands of M.Wts 11.200 and 14.800 kDa were recognized with S. manoni infected sera, one band of M.Wt 11.200 kDa was recognized with fasciola infected sera. No band detected by hydatid or ascaris or control group.