Introduction: G6PD deficiency is the most common enzymedeficiency worldwide. It is an X-linked recessive disease,affecting more than 400 million people worldwide. It causes aspectrum of diseases including neonatal hyperbilirubinemia,acute and chronic hemolysis.The aim of this work: was to screen 1000 neonates usingthe dried blood spot technique for early diagnosis of G6PDdeficiency and to asses the incidence of the disease in ourcountry before recommending using it as an obligatoryscreening test for all neoborns.Patients and methods: The study included 1000 neonates(520 males & 480 females) born in Kasr Al-Aini Hospitalbetween October 2007 and June 2008) at the first two days oflife. Screening test for G6PD was done by a heal prick using thesemiquantitative (Dried blood spot) technique and analysed inthe National Research Centre. G6PD deficient ones wererecalled again to do a confirmatory test in Abo El-Reish HospitalLab. Using the spectrophotometric quantitative enzyme assay.Results: Out of the 1000 neonates, 65 patients (6.5%) wereG6PD deficient using the screening test including 44 (68%)males and 21 (32%) females. They were recalled, but only 38were able to come and were reanalyzed. Only three maleshaving positive family history of G6PD deficiency turned to beG6PD deficient using the spectrophotometric enzyme assay.Conclusion: Using the dried blood spot technique as ascreening test, turned to be not sensitive and unreliable indetecting G6PD deficiency. So We have to look for anotherreliable and financial method for screening and early diagnosisof G6PD deficiency.