PROM occurs during the third trimester after 37 weeks of gestation. A related condition called preterm premature rupture of membranes (PPROM) occurs before 37 weeks of gestation. This prospective case-control study was carried out at Kasr Elaini Hospital. The study included 150 pregnant women between 24-36 weeks of gestation were subdivided into three groups of women. Regarding the results of urea and creatinine levels testing of vaginal washing fluid has sensitivity, specificity and accuracy of 100%, on the other hand beta-hCG sensitivity, specificity and accuracy was 83%, 100% and 85.6% respectively. In conclusion, both urea and creatinine assay and qualitative beta-hCG are best bed side tests for diagnosis of PROM than other methods but urea and creatinine level is more accurate than qualitative b-hCG test