Erectile dysfunction is inability to achieve or maintain an errection sufficiently rigid for sexual intercourse. Many studies have indicated that cigarette smoking is an independent risk factor for inducing vasculogenic impotence and the relationship between smoking and ED is independent of that between smoking and CVE. It has been focused on role transforming growth factor-Beta1 as a fibrogenic cytokine as it has been shown to increase collagen synthesis in human cavernosal smooth muscle cell by 2.5-4.5-fold. The present study was performed on (50) adult male albino rats, which were divided n (3) experimental groups; a control group that breather normal air, a group exposed to cigarette smoking for (30) days and a group exposed to cigarette smoking for (60) days. A statistically significant increase in mean value of expression of TGF-Beta1 and its type II receptor was found in comparison of the control group with the other two groups which exposed to cigarette smoking for different period of times and in comparison of the later two groups with each other. By studying the statistical correlation of expression of TGF-Beta1 and its type II receptor in the corpus cavernosum tissue of each experimental group, a statistically non significant, but direct proportionate correlation in group which exposed to cigarette smoking for (30) days and statistically significant direccty proportionate correlation in the group which exposed to cigarette smoking for (60) days was found