MBLs constitute the most clinically important group of carbapenemases, as they are capable of hydrolyzing all β-lactams, including carbapenems except the monobactam aztreonam. Acquired MBLs are emerging resistance determinants in P. aeruginosa and other non-fermentative gram-negative bacilli. They disseminate rapidly through bacterial population causing a threat to treatment with β-lactam antibiotics and carbapenems. The aim of this study was to determine the prevalence of carbapenems resistance among non-fermentative gram-negative bacilli and the prevalence of MBLs-producing isolates among carbapenems-resistant ones. Screening for carbapenems-resistant isolates among 73 non-fermentative gram-negative bacilli was done by disk diffusion method. Detection of MBLs among resistant ones was done by both phenotypic methods as IPM-EDTA combined disk method and MBL E-test and genotypic method as PCR. Results showed that the prevalence of IPM resistance among non-fermentative gram-negative bacilli was 57% with the greatest prevalence rate for P. aeruginosa 88%. The prevalence of MBL-producing isolates among IPM-resistant ones was 26% by both phenotypic and genotypic methods, while it was 74% by phenotypic methods which is a very high rate.