Recurrent pregnancy loss is one of the serious complications of pregnancy. Apolipoprotein E isoforms have been implicated in the development of this complication. The aim of this study is to evaluate the role of Apo E isoforms in inducing recurrent pregnancy loss .This study was conducted on 30 women experiencing recurrent pregnancy loss after excluding other causes and 20 healthy fertile control women. The genotype analysis involved DNA isolation followed by PCR amplification, then reversed hybridization using test strips containing allele specific oligonucleotide probes. Our results revealed that Apo E4 allele was significantly decreased in cases of recurrent pregnancy loss compared to controls while Apo E2 and Apo E3 had no significant effect.