The objective of this study was to evaluate cumulative success rates of Testicular Sperm Extraction (TESE) and Intracytoplasmic Sperm Injection (ICSI) in patients with non-obstructive azoospermia (NOA). We selected retrospectively seven hundred and sixty eight (768) patients, who had trial of testicular sperm recovery for ICSI whom were recruited from the Centre of Reproductive Medicine in the UZ-VUB hospital in Belgium during the period from 1996 to 2006. The mean age of the male was 35.6 years (21-62) and for the female was 31.6 years (18-46). Spermatozoa were found in 364/768 patients (47.4%) during TESE, only 349 patients have undergone ICSI, while the rest (15) patients did not proceed for ICSI. From total 697 ICSI cycles, fertilization was successful in 550 cycles (79%), while fertilization did not occur in 147 (21%). From the histopathological point of view, sperms were recovered in 170/380 (45%) patients with Sertoli cell-only syndrome (SCOS), 69/148 (46.6%) with tubular sclerosis, 111/204 (54.4%) with maturation arrest, in 1/1 (100%) patient with Carcinoma insitu and in 10/35 (28.6%) patients with no available testicular histopathology, while delivery occurred after ICSI in SCO patients 55/170