Asymptomatic bacteruria in pregnancy is defined as the presence of a significant amount of bacterial growth in a urine culture taken from a urine sampleand the absence of symptoms of urinary infection such as pain or urgency. The aim of this work is to assess the prevalence of asymptomatic bacteruria in pregnant ladies attending Bani-Sueif University Hospitals, obstetrics and gynecology outpatient clinic. All patients were followed up through 2 visits: 1st visit (6th-10th weeks) &2nd visit (22nd-24th weeks).Then follow up visit for 2nd culture after the initial treatment and another course of antibiotic given for those still positive.All patients with ASB were classified into 2 groups: Group (1):received single dose antimicrobial agent according to the result of culture and sensitivity test e.g. Amoxicillin-Clavulinic Acid/ Nitrofurantoins/ Cefalexin). Group (2): received multiple doses antimicrobial agents according to the result of culture &sensitivity test for 3 days. Then culture and sensitivity repeated one week after the course of antibiotic is finished and the results of the two groups were compared. All antimicrobials were chosen according to the result of culture &sensitivity with safety of use during pregnancy assured. The antibiotic sensitivity patterns showed that most of the bacterial isolates were sensitive to amox-clav, cephalexin, and nitrofurantoin. There are different regimens in treating asymptomatic bacteriuria during pregnancy, of them single day course and three days course.