Blastocystis hominis is a unicellular organism found commonly in the intestinal tract of humans and many other animals. Very little is known about the basic biology of this organism. The common morphological forms are vacuolar, granular, and amoeboid. Other additional forms were recently detected. Infections with the organism are worldwide and appear in both immunocompetent and immunodeficient individuals. Gastrointestinal symptoms generally attributed to blastocystosis infection. In the present study detection of this organism was done in Beni Suief University Hospital by using different diagnostic techniques which gave different variations in the obtained results.