Both flow cytometry and static image analysis have been used to determine DNA content (ploidy) of prostate lesions. Quantitative DNA variables were evaluated in prostatic core biopsies from patients with various prostatic lesions. An increase of DNA content was observed from normal to cancerous tissues. Objective: The goal is to evaluate the role of the image analyser system in differentiation between benign, precancerous, and malignant prostatic lesions. Also, a correlation will be done between the morphometric and DNA ploidy data. Study design: The present study included 60 prostatic core biopsies consisting of 20 cases of benign prostatic hyperplasia (BPH), 20 cases of prostatic intraepithelial neoplasia (PIN), 20 cases of prostatic carcinoma and 5 cases as a control. DNA content analysis of sections stained with blue Feulgen stain and nuclear morphometrical analysis of the haematoxylin and eosin stained sections were carried by using the Leica Qwin 500 image analyzer. Result: All benign lesions included in this study were diploid (2C) and all the malignant lesions were aneuploid (>4C) except one case which was diploid. The diploid DNA value was significantly higher in the normal group than in all other groups (p=0.000); and lower in the carcinoma group than in all other studied groups (p=0.000). Also the diploid value was significantly lower in PIN group than in BPH groups (p=0.000). The proliferation index (S-phase) value was significantly higher in all groups when compared to normal (p<0.008). The aneuploid value was significantly higher in carcinoma group than in all other studied groups (p=0.000). The normal group did not show any aneuploid values. The mean nuclear area in carcinoma group was significantly higher than that of normal, BPH and PIN groups (P=0.000).Conclusions: The DNA ploidy analysis helps to differentiate between normal, BPH, PIN and malignant groups. Distinction between PIN and cancer could be approached on more objective criteria by DNA and nuclear morphometrical analysis.