This study aimed to demonstrate the effect of omega-3 fatty acids supplements on serum IL-1 and TNF-α concentrations in JIA patients, clinical manifestations, laboratory investigations and on daily requirements of NSAID.Fifty five participants were included in this study; they were divided into two groups:Group 1: Thirty five Juvenile Idiopathic Arthritis patients were included for this study, diagnosed according to ACR classification from the Rheumatology and Rehabilitation outpatient clinic, and Abu-El Rish Children hospital, Faculty of Medicine, Cairo University Hospitals. Only 24 patients continued the study (took omega-3 for 12 weeks), the rest of patients were excluded from the study due to irregular attendance. Group2:Twenty healthy volunteers serving as control group.All patients included in this study were subjected to full history taking, clinical examination, laboratory investigations .All JIA patients took dietary supplements of omega-3 fatty acids in the form of capsules (500mg/capsule), 4 capsules/day in two divided doses for 12 weeks, each capsule contains 500 mg omega-3 Fatty acids (300 EPA, 200 DHA) There is statistically significant decrease in serum IL-1 and TNF-α after dietary supplement of omega-3 fatty acids in JIA patients and also significant difference in comparing with control group.There is significant improvement in DAS-28, VAS, CHAQ and laboratory investigation. There is decrease in the doses of NSAID along the course of treatment.