Premarital care is a worldwide activity aims to diagnose, treat unrecognized disorders, and reduce transmission of diseases to couples. The study was an interventional educational prospective study; its main concern was to assess and improve knowledge and attitude of 200 medical students in Fayoum University towards premarital care services; through health education intervention in the form of lecture and brochures that addresses important issues of premarital care. The involved students were interviewed by using closed-ended questionnaires, which was distributed twice, just before the intervention and after 2 months of its date to detect the degree of knowledge remained. Eighty one percent of the sample was less than 20 years old. Males represented (40%) of it. Total knowledge score showed significant improvement from (62.44±4.98) to (69.37±3.43), especially knowledge about premarital counseling session content score increased from (9.84± .776) to (11.63± .766), knowledge about role of PMC in prevention of STDs as gonorrhea and hepatitis increased up to 95% and 72% respectively, and knowledge about premarital vaccination increased from 35.5% to 74% . Total attitude score showed significant improvement from(7.89±1.1) to (13.1±.81) especially attitudes towards importance of PMC services, convention to receive such services, and refusal of marriage person with untreated infectious diseases with percents up to94%,92.5% and90% respectively after application of the health education intervention. However, females showed better attitudes than males after improvement of their knowledge about PMC. The results reflected the importance of health education as a cornerstone element in improving knowledge and attitude towards different public's concern. The need of continuous health education programs for students to get benefits from their changed positive attitudes towards such services as they will become the future physicians.