Purpose: Assessment of macular thickness and retinal nerve fiber layer thickness by time domain stratus OCT and detection of its relation to visual field loss. Patients and methods: A total of 45 eyes, 15 control cases and 30 cases with primary open angled glaucoma (P.OAG) were included in the study and subjected to visual acuity, clinical examination gonioscopy, IOP measurements, automated perimetry (SAP) central 30-2 and time domain stratus measurements of macular and RNFL thickness and statistical analysis was done for all the results. Results: There was statistically significant difference in average and quadrantic RNFL thickness, Macular thickness (TOM) quadrant (IOM) quadrant, between glaucoma cases and control cases. There was statistically significant positive correlation between average (RNFLT) and mean deviation and also between mean macular thickness (total average macular thickness) and mean deviation with P value (0.000) for (av. RNFLT) and (0.01) for (mean M.T). There was no statistically significant difference in average foveal thickness between glaucoma cases and control cases. There was statistically significant positive correlation between average (RNFL) thickness and mean macular thickness in glaucoma cases and control cases with P value (0.000). Conclusion: Time domain OCT has a good accuracy in assessment of RNFL thickness and macular thickness measurements. Combination of (OCT) RNFL thickness, average and quadrantic especially inferior Q. and temporal Q. plus macular thickness (TOM) and (IOM) and average plus field of vision mean deviation are of high accuracy for assessment and diagnosis of (POAG). Recommendations: OCT Macular thickness measurements should be interpreted only under the context of refractive error, age and location of measurements. Combination of OCT (Retinal nerve F.L) thickness and macular thickness temporal outer quadrant, inferior outer quadrant and average plus field of vision mean deviation are of high accuracy for assessment and diagnosis of (POAG) primary open angle glaucoma. Scan centralization is very important to obtain good results.