Brucellosis is still an endemic serious disease among domestic animals and human, constituting a public health problem in Fayoum Governorate; hence this descriptive study was carried out to expose the existing knowledge, attitude and practices of the available direct animal contacts towards brucellosis in Fayoum governorate. A total of 300 persons of direct animal contacts were interviewed by using questionnaire form. The findings from the study revealed that, the general awareness about brucellosis was low (about 49.7% of the participants not heard about brucellosis) most of them were animal breeders and abattoir workers. There was significant difference between the 3 groups regarding knowledge (p value=0.000) with higher mean score among animal examinators. The attitude toward preventive measures of brucellosis was positive. Good knowledge and positive attitude especially from veterinary doctors not always translated into sound practices, mainly due to lack of supplies (masks, gloves, coat and vaccines). The general practices of animal breeders regarding, cleaning, disposal of animal waste and vaccination of animals was bad.