Introduction : Jaundice is the most common condition requring attention in newborn. It is commonly due to accumulation of unconjucated bilirubin, mostly it refelects normal transitional phenomena. However in some infants it may lead to life long neurological sequelae or death.G6PD deficiency is the most common enzyme deficiency worldwide. It is an X-linked recessive disease, affecting more than 400 million people worldwide. Common complication of G6PD is severe neonatal Hyper bilirubinemia with the potential of kernicturs. The aim of this work : is to detect cases of G6PD deficiency among newborns with indirect Hyperbilirubinaemia in our country before recommending it as an obligatory screening test for all newborns using the quantitative method and for early diagnosis of G6PD deficiency.Patients and methods: This study was conducted on 200 hundred full term neonates with neonatal jaundice, 158 males and 42 females. They were selected from the NICU in Beni-Suef University teaching hospital.Cases were recruited during the study period from May 2010 to January 2011.Results : Out of the 200 neonates , 8 patients (4%) were G6PD deficient using the screening test.Conclusion : despite low incidence of G6PD deficiency in our study , we recommend screening for G6PD deficiency in any neonate presenting with jaundice or any neonate with positive family history for G6PD deficiency not only to detect the etiology of jaundice, but also te prevent kernicterus and future haemolytic episodes.