Helicobacter pylori plays a pathological role in gastrointestinal diseases through excessive reactive oxygen species production, pronounced membrane damage and the depletion of antioxidants. In the study, we measured the levels of natural antioxidants, namely Superoxide dismutase (SOD), Reduced Glutathione (GSH) as well as Malonyl Dialdehyde (MDA) as markers of oxidative stress. The study included five groups, a contol group of 10 patients, 10 H pylori +ve duodenal and/or gastric ulcer patients receiving proton pump inhibitors only (Group I), 10 H pylori +ve duodenal and/or gastric ulcer patients receiving proton pump inhibitors +antibiotics(Group II), 10 H pylori +ve duodenal and/or gastric ulcer patients receiving proton pump inhibitors +antibiotics+antioxidants(Group III), 10 H pylori -ve duodenal and/or gastric ulcer patients receiving proton pump inhibitors antioxidants(GroupIV). We found that SOD and Reduced Glutathione were significantly reduced in all groups as compared to the control group. MDA was significanly higher all groups as compared to the control group. We recommend antioxidant supplementation to patients with peptic ulcer or gastritis to aid in recovery from oxidative stress and to guard against gastric malignancies.