Tc-99m sestaMIBI is a myocardial perfusion imaging agent and has tumor imaging capabilities .It is known among its normal biokinetics that its main excretory route is through hepatobiliary tree. In our study Tc-99m MIBI was used to evaluate its role in diagnosis of Ch CC in comparison with IDA derivatives besides evaluation of its normal biokinetics in adults . The study revealed that Tc-99m MIBI can be successfully employed in assessment of patients with Ch CC . The main diagnostic criteria for diagnosis of Ch CC using Tc-99m MIBI are GB nonvisualization, GB delayed visualization more than 30 minutes and diminished GB contractility with low GBEF less than 50% . Finally this study is considered a screening test which need more studies to confirm this criteria.