Acute myeloid leukemia (AML) is a clonal disease of haematopoietic tissue that is characterized by proliferation of leukemic blast cells in the bone marrow and impaired production of normal blood cells. (Litchman and Liesveld, 2001). Cytogenetic studies revealed recurring abnormalities of which one of the most common is translocation (8;21). (Hromas et al., 2001). This work is performed on 15 newly diagnosed cases of AML M2 in order to study the incidence of T(8;21) among AML M2 patient using fluorescence in situ hybridization (FISH) technique. T(8;21) was found in 20% of adult cases and 40% of pediatric cases of AML M2. FISH analysis of T(8;21) with the AML1 and ETO probes is extremely valuable in cases of AML M2 due to its high ability to reveal T(8;21) (q22;q22) and this confirm the diagnosis allowing patients to be assigned to the correct risk group in terms of treatment.