The aim of this work was to evaluate the effect of L-carnitine and zincmedication on the DNA fragmentation of ejaculated sperm using the spermchromatin dispersion test.Twenty five patients seeking fertility were recruited from KasrElEiniandrology clinic. Their age ranged from 20 to 46 yrs old and infertility periodranging from a 4 months period up to 19 years. The first semen samples werecollected, processed for the sperm chromatin dispersion test, then stained withDAPI & read by fluorescent microscope. Fifteen patients continued theirfollow-up and their second semen samples were collected and processed in thesame manner after completing one month treatment with L-carnitine 3gm/dayand Zinc gluconate 150 mg/d.The mean for DNA fragmentation index before treatment was 39.17 %,(± SD 36.99%). The mean for DFI after treatment was 45.5% ( ± SD 28.47%).The Wilcoxon signed ranks test showed a very high p-value of 0.65 implyingthat there was no difference in the level of DFI before and after the treatment.By selecting the patients with an initial DFI above 30 we would find that theirDFI improved after treatment from a mean of 71.01% (± SD 22.63%) to48.14% (± SD 26.59%) , yet this trend in the improvement was alsostatistically insignificant according to the Wilcoxon signed ranks test.No correlations were found between DFI and either sperm count, abnormalforms, motility and progressive motility.