This study has been conducted on 40 adult male and female albino rats to determine the histological features in the odontogenic tissues of developing lower first molar of albino rats with immunohistological localization of tenascin-C and integrin β1 in the same tissues at different stages of its development and along its pathway of eruption. Three females to one male rat were caged together and left for mating. When pregnancy was determined by pregnancy test, each pregnant rat was caged separately waiting for delivery. The offsprings were sacrificed by cervical decapitation at the following ages: at birth (Group I), one week (Group II), two weeks (Group III), three weeks (Group IV), four weeks (Group V). The results showed that, mild staining reactivity for tenascin-C appeared in most of the odontogenic tissues at birth and the reaction increased markedly at age of one week. At age of two weeks, the reactivity to tenascin-C decreased mostly in the odontogenic tissue and further decrease in tenascin was observed at the age of three weeks. The expression increased again at the age of one month by the eruption of the tooth. Integrin expression in the odontogenic tissues at birth presented mostly weak to mild intensities with a slightly greater reactivity in developing bone trabeculae and associated bone marrow. The reactivity to integrin decreased as the tooth germ grew from birth to one and two weeks and then increased slightly at the age of three and four weeks particularly in the bone marrow, interstitial tissue of periodontal ligament and in the zone of intermediate plexus cervically. It has been concluded that, there was a positive correlation between immunohistochemical localization of tenascin-C and integrin β1 in the odontogenic tissues of lower first molar of albino rats and the processes of initiation, proliferation, cellular differentiation, apposition and tooth movements.