Background: Recently, Platelet-rich fibrin (PRF) was described as a second-generation platelet concentrate.PRF is known as a rich source of autologous cytokines and growth factors and universally used for tissue regeneration in current clinical medicine. Objective: Radiographic evaluation of the effect of platelet rich fibrin (PRF) on the quality and the quantity of bone formation in unilateral maxillary alveolar cleft reconstruction. Patients and methods: Twelve patients with unilateral alveolar cleft underwent reconstruction; Group I includes six patients grafted with PRF combined with autogenous anterior iliac crest bone graft (AIC). Group II includes six patients were grafted using autogenously AIC bone graft alone (control group). Computed tomography (C.T.) was made to assess the quality and quantity of the newly formed bone. Results: sufficient bone bridging was achieved in both groups, The percentage of newly formed bone(quantity) in group A ranged from 79.74% to 88.4% with mean percentage of 82.6 %±3.9 %.While in group B, The percentage of bone formation ranged from 60.3 % to 76.4 % with mean percentage of 68.38 %± 6.67 %, the statistical results showed that there was a statistically significant increase in percentage of the newly formed bone in group A (PRF+AIC) than in group B (AIC), The mean bone density(quality) of the newly formed bone of group A was found to be lower than that of group B but not statistically significant, p=>or<0.05. Conclusions: PRF in combination with autogenous bone was beneficial in improving the volume of the newly formed bone in alveolar cleft reconstruction.