Objectives: To investigate the effect of different self-etch adhesive systems application techniques: active or passive in a single or double layer on adhesive-dentin microshear bond strength. Methods: Occlusal surfaces of 64 extracted human molars were ground to expose a flat superficial dentin surfaces. Specimens were randomly divided into two main groups according to the tested self-etch adhesive system either: One-step self-etch (AdperTM easy-one) or two-step self-etch (AdperTM SE Plus). Each adhesive system was applied on the prepared dentin surfaces followed one of these techniques: 1) Passive application of a single layer, 2) Active application of single layer, 3) Passive application of double adhesive layer (with light curing in between) and 4) Active application of double adhesive layers. Resin composite was packed inside micro-tubes fixed on the bonded dentin surfaces and light cured for 40 seconds. All specimens were stored in artificial saliva either for 24 hours or 3 months before testing. Microshear bond strength test was employed using a universal testing machine at a crosshead speed of 0.5mm/min. Ultra-morphological evaluation for dentin-adhesive interface was tested using scanning electron microscope. Results: AdperTM SE Plus showed higher significant microshear bond strength in compared with AdperTM easy-one. For both adhesive systems active application showed higher significant microshear bond strength to dentin than passive application. Double application of adhesive systems showed lower microshear bond strength than single application. Conclusion: Active application of self-etch adhesives could be able to improve the dentin microshear bond strength. Double application with curing in between the layers didn't improve the bond strength to the tested adhesive.