Aim: This study was designed to evaluate the antibacterial activity, microtensile bond strength and the effect of aging of three different self- etch adhesives; one containing antibacterial agent, Clearfil protect bond (PB), one without antibacterial agent, Clearfil SE bond (CS) and one containing fluoride, Futurabond NR (FB). Methods: For the antibacterial activity study a total of 20 dentin specimens for each adhesive system were prepared with 6±0.5mm length and 2±0.5mm thickness, and then adhesive systems were applied according to the manufacturer instructions. Specimens were then placed in the wells of Streptococcus mutans, and then the diameters of the inhibition halos around the materials were measured in mm for the 24 hours and the 3 months aging. For the microtensile bond strength composite-dentin sticks with a cross-sectional area of approximately 1mm2 were obtained after 24 hours or 3 months aging. They were subjected to microtensile bond strength testing at a cross head speed of 1.0mm/min. Statistical analysis was done using one way ANOVA followed by post hoc test for comparison (P≤ 0.05). Results: The highest antibacterial activity was recorded for the Clearfil Protect bond while the lowest was for Futurabond NR. These records were after 24 hours, however after 3 months there was not any tested adhesive recorded antibacterial activity. For the microtensile bond strength results, the highest value was recorded for the Clearfil SE adhesive after 24hours while the lowest value was for the Clearfil protect adhesive after 3 months. Conclusions: Incorporation of antibacterial agent could be a potential method for providing self-etching adhesive with an antibacterial activity. On the other hand the microtensile bond strength might be affected by the addition of the antibacterial agents. Aging could have a detrimental effect on the antibacterial activity of the self etching adhesives while its effect on the microtensile bond strength might be material dependant.