Objectives: The research was designed to study the effect of temperature whether refrigerated or ambient on the micro-shear bond strength of a single-step self-etch adhesive systems to enamel and dentin. Methods: Sixty human upper central incisors were used, after preparation of the labial surfaces the iBond, Bond Force and Adper Easy-One adhesive systems were applied at both temperatures; refrigerated (5oC±1) or ambient (24oC±1) and then the resin composite (Tetric Evo-ceram) was applied. The prepared micro-cylinders were then subjected to micro-shear bond strength testing in a universal testing machine and the results were statistically analyzed using Student's t-test. Results: No statistically significant difference was found between both adhesive temperatures. Conclusions: The use of single-step, self-etch adhesive systems at refrigerated or ambient temperatures achieve comparable bonding to tooth substrates.