This study was carried out to evaluate the bond degradation resistance of the Self Adhesive Flowable Resin Composite as assessed by nanoleakage comparing it to different types of resin composite restorative materials, namely Flowable and Micro-Hybrid ones with and without thermocycling, and at different storage periods of one week, and three months. A total of 120 intact permanent human molars were selected for this study. Standardized class V cavities were prepared on the buccal surfaces of the teeth. The teeth were randomly divided into 3 groups according to the type of the restorative material used. Half of the restored teeth of each group were thermocycled. The thermocycled and the non-thermocycled restored teeth were stored in distilled water according to the aforementioned storage periods. Each tooth was sectioned into 2 halves longitudinally in a bucco-lingual direction through the center of the restoration. Freshly prepared ammoniacal silver nitrate solution was used for nanoleakage assessment. Each sectioned tooth was examined by quanta environmental scanning electron microscope using backscattered electron mode to evaluate the nanoleakage and to show clearly the silver deposition within the formed hybrid layers and within the resin/dentin interfaces. The quantitative analysis for the component elements at the selected spots (occlusally and cervically) at the resin/dentin interfaces were carried out using the Electron Dispersive Analytical X-ray (EDAX) of the Environmental Scanning Electron Microscope (ESEM). The recorded data were collected and statistically analyzed. Results revealed that the Micro-hybrid composite showed the statistically significantly lowest mean silver penetration values, followed by the conventional Flowable composite, while the Self adhesive resin composite showed the highest mean silver penetration values. Thermocycled specimens, and specimens stored for 3 months showed statistically significant higher mean silver penetration values than non-thermocycled specimens, and specimens stored for 7 days respectively. It was concluded that nanoleakage is a dynamic phenomenon which is affected by the restorative material composition, storage time and thermocycling.