This study was carried on to measure and compare the rate of Fluoride release from Zinc reinforced glass ionomer cement and conventional one at different time intervals namely; one week, and one month, measure and compare the amount of Fluoride and Zinc uptake by dentin at the same selected time intervals and to investigate whether leaching out of Fluoride and Zinc from Zinc reinforced glass ionomer cement could affect its shear punch strength at the aforementioned time intervals or not. Fluoride release measurement was done using a specially constructed split Teflon mold to prepare the specimens. The mold was 2mm thick and has a 3 circular holes with internal diameter of 3mm. Twenty specimens were prepared, they were divided to two equal groups according to the type of the restorative material used (A), ten discs for each tested group; in the first group (A1) conventional glass ionomer material (Chemfil Molar) was used, in the second group (A2) zinc reinforced glass ionomer material (Chemfil Rock) was used. For each group, the cumulative amount of Fluoride release was assessed; at first day, one week, two weeks, 3 weeks, and four weeks using an ion analyzer. Fluoride uptake, a total of 60 intact sound freshly extracted human permanent posterior teeth (extracted for periodontal reasons) were selected for the study.Standardized class V cavities were prepared on the buccal surfaces of selected teeth. Each class V cavity was restored with its corresponding restorative material according to the manufacturer’s instructions. The selected teeth were divided into 3 equal groups according to the materials used (A1, A2 and A3). In group A1, the cavities were left without restoration (control group). In group A2, conventional Glass ionomer was used. In group A3, conventional glass Ionomer material was used. Each group was divided into 2 equal subgroups according to the storage period (B1 and B2). In subgroup B1, the specimens were stored for one week in artificial saliva. In subgroupB2, the specimens were stored for one month in artificial saliva. Specimens were sectioned bucco-lingually and they were subjected to scanning electron microscope with EDAX analysis to evaluate the amount of Fluoride uptake by the tooth. Shear punch strength test, was done by using specially constructed mold which was fabricated to prepare specimens from the tested materials. Twenty specimens were prepared from each material. They were subjected to shear punch test to measure the shear punch strength of each tested material after one week and one month. Data were collected, tabulated and statistically analyzed using ANOVA and Tukey HSD. Results of the study: 1. Conventional glass ionomer represented by ChemFil Molar showed higher fluoride release than zinc reinforced glass ionomer represented by ChemFil Rock. 2. Conventional glass ionomer represented by ChemFil Molar showed higher fluoride uptake by dentine than zinc reinforced glass ionomer represented by ChemFil Rock. 3. Zinc reinforced glass ionomer represented by ChemFil Rock showed higher shear punch strength than conventional glass ionomer represented by ChemFil Molar. Therefore, under the circumstances of this study the following conclusions are drawn: 1. Fluoride release from glass ionomer restorative material is a material dependent property. 2. Fluoride content and release from glass ionomer restorative material have an influential effect on its amount being up taken by dentin. 3. Zinc reinforcement of glass ionomer restorative material is a successful method for improvement of its shear punch strength