Objectives: was designed to investigate the effect of different applications of ethanol wet bonding technique (one, two, three applications or ethanol washing) on bond strength durability of three-step, etch-and rinse adhesive system bonded to dentin under simulating intrapulpal pressure and aged for 24 hours and six months at 37oC. A total number of 100 sound human third molar teeth were randomly divided into five equal groups, of 20 teeth each, according to substrate condition (A); (A1): water wet bonding technique (control), (A2): one application of 100% ethanol, (A3): two applications of 100% ethanol, (A4): three applications of 100% ethanol and (A5): ethanol washing. Each group was subdivided into two equal subgroups, of 10 teeth each, according to the adhesive system utilized (B); where (B1) hydrophilic adhesive resin (with primer) and (B2) hydrophobic adhesive resin (without primer). Each subgroup was further classified into 2 classes, of 5 teeth each, according to the aging period (C); where (C1) specimens were tested after 24 hours and (C2) after 6 months storage in artificial saliva under simulated intrapulpal pressure. Standardized mid-coronal dentin surfaces were prepared perpendicular to the long axis of the teeth after removal of occlusal enamel. All dentin surfaces were etched for 15 seconds with 35% phosphoric acid and rinsed with water for 15 seconds. AdperTM Scotchbond Multi-Purpose adhesive system was applied either with / without primer followed by composite buildups using FiltekTM Z250 XT. All aged specimens were sectioned into sticks for micro-tensile testing, failure mode analysis and nanoleakage assessment. Three-way ANOVA test showed no statistically significant difference between control and two ethanol applications; both showed the statistically significantly highest mean micro-tensile bond strength values. Ethanol wash showed the statistically significantly lowest mean micro-tensile bond strength. The 24 hours period showed statistically significantly higher mean micro-tensile bond strength than 6 months period. At 24 hours groups, the most predominant type of failure was type II mixed failure while at 6 months groups; type III mixed failure was predominant. Nanoleakage analysis showed silver staining but with different degrees of deposition and forms.Conclusion: Ethanol wet bonding technique enables the use of hydrophobic resin for dentin bonding. None of the tested ethanol-wet bonding protocols is reliable in terms of bond strength durability.