Aim: This study was carried out to investigate the effect of Er:Yag laser ablation on the bondingefficacy and durability of ceramic blocks to enamel and dentin using contemporary resincements.Materials and methods: Half of exposed flattened labial and occlusal surface of 60 uppercentral teeth and 60 molar teeth respectively were marked with a permanent felt-tip marker.Subsequently, these marks were erased using high speed cylindrical carbide bur # (C21.314.008)(EDENTA AG, Switzerland) in bur-prepared groups (B). The other half was marked and themarks were erased using an Er:YAG laser (Fidelis, Fotona ) in laser-ablated groups (L). Theprepared teeth were divided into three groups of 20 teeth each according to the type of resincement used to lute 3mm thick ceramic sections; either conventional resin cement withpretreatment with either etch-and-rinse (E&R) or self-etch (SE) adhesive or the direct use of selfadhesivecement (SA). After cementation, the teeth were stored in artificial saliva then half of thespecimens from each subgroup (10 teeth) were tested after 24 hours (I). The other half was agedby storage for six month (A). 7 teeth from each subgroup were randomly selected for the microtensilebond strength (μTBS) test. Meanwhile, the remaining 3 teeth were allocated for the ultramorphologicalanalysis.Results: In the enamel group, laser ablation didn’t influence the bonding performanceof (E&R) and (SE) at both 24 hours and 6 months storage in artificial saliva. Moreover, when(SA) was used, there was no statistically significant difference between (μTBS) of B and Linitially. Meanwhile, laser ablation improved the bonding ability of (SA) after aging.Furthermore, with the three tested resin cements, the results indicate no degradation of (μTBS)after aging with B and L. Moreover, (E&R) exhibited the highest bond strength to B and L. Forthe dentin group, there was no statistical significant difference between (μTBS) of B and Lgroups with both (E&R) and (SE) systems after 24 hours storage. Meanwhile, after aging,(μTBS) of B (E&R) and B (SE) groups significantly decreased. On the contrary, L groupsrestored with (E&R) and (SE) were not affected by aging and their (μTBS) remained stable after6 aging. (SA) cement showed significant higher (μTBS) to L when tested after 24 hours. On theother hand, its bond strength to B significantly increased after aging, to become statisticallysimilar to L, while the bond strength of the same cement to L remained stable after aging.Clinical Significance: Utilizing Er:Yag laser for tooth preparation is a viable alternative thatenhances durable bonding of indirect machinable ceramic restorations.