Objective: Evaluate the antibacterial activity, elemental composition and bond strength test of silver nanoparticles incorporated in dentin adhesive material. Materials and Methods: Adper scotch bond multipurpose adhesive was the material of choice where different concentrations of spherical silver nanoparticles having a size of 4 nm and ranging from 2.5, 5 and 10 were used to modify it. Twenty sound non-carious molars were assigned randomly for the micro-shear bond strength test. Modes of failure were distinguished by scanning the fractured specimens with an environmental scanning electron microscope. For assessment of antibacterial activity, direct contact test was performed and the viability of the bacteria was investigated using the fluorescent microscope. Finally, the tooth adhesive interface was analyzed with the EDX machine for elemental composition analysis. Results: The bond strength of the material and the modes of failure did not show any statistical significant difference between the control and the experimental material. Adding silver nanoparticles to the resin adhesive altered its biological activity rendering it a material expressing antibacterialaction which is directly proportional to the concentration. For viability assessment, the image analysis of the fluorescent microscope pictures showed that the 10 had bactericidal effects on the Streptococcus mutans whereas the 5 had bacteriostatic effect. Both are opposite to concentration 2.5 that did not show any statistical significant change change from the control. The EDX analysis proved the presence of silver in the hybrid layer. This means that incorporations of small sized Nano-silver in the material will subsequently lead to its flow in the dentinal tubules along with the resin adhesive. The silver will be in direct contact with the tooth and any residual bacteria in the cavity or bacterial ingress from the microleakage will be subjected directly to the silver effect.Conclusions: This study concludes that incorporation of antibacterial silver nanoparticles into a dentin adhesive is an essential factor in inhibiting residual bacteria in the prepared cavity and secondary caries. Furthermore, this action is concentration dependent; it increases with the increase of silver addition and varies from bacteriostatic to bactericidal effect according to the amount added. However, lower concentrations do not have any effect on the material regarding its antibacterial activity. It is important to note that modifying a dentin adhesive with silver nanoparticles within this current study did not have any effect on the bond strength of the material.