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Genetically induced bone grafting material as a substitute for harvested autogenous bone graft in bone defects: Experimental study

Thesis

Last updated: 06 Feb 2023

Subjects

-

Tags

Oral & Maxillofacial Surgery

Authors

Mahmoud, Marwa Ragaei

Accessioned

2018-08-26 05:48:04

Available

2018-08-26 05:48:04

type

M.Sc. Thesis

Abstract

Craniofacial tissue engineering promises the regeneration or de novo formation of craniofacial structures lost due to congenital anomalies, trauma, or diseases. Bone is one of the important structures to be regenerated. The generation of induced pluripotent stem cells provides the opportunity to use patient-specific somatic cells, which are a valuable source for disease modeling and drug discovery. To promote research involving these cells, it is important to make induced pluripotent stem cells from easily accessible and minimum invasive tissues, like blood and that is what was done in our study, we used peripheral whole blood to induce white blood cells to a pluripotent state. Blood-derived induced pluripotent stem cells are indistinguishable from embryonic stem cells with respect to morphology, expression of surface antigens, and pluripotency-associated transcription factors, and DNA methylation status at pluripotent cell-specific genes . In this study our aim was to genetically induced bone grafting material as a substitute for harvested autogenous bone graft in bone defects. More research is to be carried to test this cell line from peripheral blood to induce fibrogenesis, chondrogenesis or osteogenesis.

Issued

1 Jan 2013

DOI

http://dx.doi.org/10.21473/iknito-space/40023

Details

Type

Thesis

Created At

28 Jan 2023