Objective: This study aimed at evaluating motor nerve regeneration using stem cells inside guided tubes for the purpose of developing a rationale for a minimal morbidity promising procedure with most acceptable cosmetic and functional results for patients with Facial Nerve affections.Materials and methods: Nine adult mongrel dogs were included in this study, Animals were generally anaesthized and a gap of 3 cm was made on the buccal branch of right side of facial nerve and also on the left side as a “control side”, Total population of mononuclear cells containing mesenchymal stem cells were separated from whole bone marrow using Gradient separation method in which cells are separated according to the cell size using Ficoll. Silicone tubes embedded with collagen type I sponge seeded with stem cells and neurogenic media “containing nerve growth factors” were sutured in the perineural fascia of both ends of the cut nerve on the experimental side, while in the control side empty silicone tubes alone were sutured to the perineural fascia of the cut nerves ,healing was evaluated at 6 ,8 and 10 weeks scarifying 3 dogs each time and performing histopathological analysis. Results: All dogs after 6 weeks showed no observable muscle movement and increased drooling although the entire subjects at the experimental side showed significant wound healing with limited scaring. After 8 weeks the remaining dogs (6 dogs) showed limited movement in the facial area supplied by the buccal nerve and the salivary drooling decreased moderately. After 10 weeks the remaining dogs (3 dogs) showed observable muscle movements and decreased salivary drooling in contrast to control side. These results go with the histological examination results showing massive proliferation of undifferentiated cells at 6 weeks then multiple blood vessels formation and nerve like structures with partial absorption of the scaffold at 8 weeks then well organized nerve like tissues with dilated blood vessels at 10 weeks.Conclusion: collagen scaffolds seeded with mononuclear layer containing stem cells and nerve growth factors showed efficacy in regenerating facial nerve.