The literature lacks evidence regarding the determination of mandibular axis of rotation in Skeletal Class II and III subjects. This study was aimed at finding this axis. 32 subjects were recruited and CBCT scans of the full face were obtained; while wearing interocclusal splints, along with scanned dental digital models. These were used and registration was done to find the center of rotation using 3D softwares. The results showed that there was high variation in the position of the axis. This could be due to variation in individual’s anatomy. ResultsThis study has introduced results worthy of further inquiry. It was not possible to group axes of rotation found due to the high variability in results. No statistically significant difference was found between the axis in the right and left side of the mandible in Class II or Class III subjects except the anteroposterior measurements in the Class II group where high significant difference was found. Moreover, No correlation exists between the vertical distance and horizontal distance in both sides of the two groups.Conclusions CBCT imaging and digital models were valuable tools that helped in finding the mandibular hinge axis of rotation in Skeletal Class II and III subjects. No common axis of rotation was found in Class II subjects, but rather different axes were found.No common axis of rotation was found in Class III subjects, but rather different axes were found.Mandibular axis of rotation of Class II and III subjects should be considered separately for each patient when orthognathic, prosthodontic or restorative procedures are needed.