This study aimed to identify the risk of bacteremia in pediatric patients during dental treatment under general anesthesia procedures, and also to determine the circulating microorganisms in their blood.The study group was composed of forty patients, undergoing dental treatment under general anesthesia. Peripheral venous blood samples were collected at baseline and at 2-5 minutes after completing different dental procedures. Manual blood culture system was used in this study. The blood samples were subcultured after 2,4,6,8 and 10 days in MacConkey agar plates to identify the different types of bacteria isolated.The prevalence of bacteremia at the baseline was 0%, while there were 11 patients representing (27.5%) showed positive blood cultures after completing dental procedures.The bacteria most frequently identified in the positive blood culture were Staphylococcus species (81.8%), particularly Staphylococcus aureus.Conclusions: In the present study, bacteremia was detected after dental procedures in pediatric patients who were treated under general anesthesia.