Purpose: To isolate and culture of stem cells extracted from human dental pulp of an exfoliated primary teeth and to evaluate the effect of Emdogain®-gel on the culturing cells after isolation from human dental pulp of the primary teeth.Materials and Methods: (First stage), dental pulp stem cells have been isolated from the pulp tissue of human primary teeth using digestion method.(Second stage),evaluation of cell proliferation of human dental pulp stem cells from primary teeth were divided to two groups of dishes each (control group.1)which contain UDMSCs (undifferentiated mesenchymal stem cells) in culturing media without containing extracted enamel proteins other group of dishes(experimentalgroup.1) contain UDMSCs culturing media with extracted enamel proteins.(Third stage),evaluation of differentiation and mineralization of human dental pulp from primary teeth was investigated through two selective groups of dishes were (control group.2) there were containing UDMSCs without osteogenic media and other group(experimental group.2) the experimental group which contains UDMSCs with Emdogain and osteogenic media.Results: Human total primary dental pulp stem cells were successfully isolated from the pulp of the human teeth and began to attach to the bottom of dishes. The cells appeared with various moropholgies, reflecting the diversity of pulp cells.The cells continue to proliferate and propagate until 90% of the dish area was covered with cells by the seventh day to the tenth day indicating a highly proliferative cell population in the dental pulp. The success in evaluation of biological effect of emdogain at concentration 100μg/ml was measured by the increase in number of cells in primary culture stage, then by measuring calcification areas stained by alizarin red which indicates large areas of mineralized tissue performed by odontoblast-like cells.Conclusions: Emdogain® is a bio-inductive material that is compatible with vital human tissues. It offers a good healing potential and is capable of inducing dentin formation, leaving the remaining pulp tissue healthy and functioning.