The purpose of this study was to evaluate the antimicrobial activity of root canal irrigants namely propolis extract, QMix, chloroxylenol and chlorhexidine plus compared with NaOCl 5.25% (positive control) and saline (negative control). : Enterococcus faecalis (batch no. 10541) thawed and incubated under aerobic condition. The harvested bacterial colonies are then spectrophotometrically calibrated to 5.9 x 104 (CFU/mL-1). Fifty specimens instrumented conventionally, reaching master apical file size #50. Twenty μL of the bacterial culture transferred to the canal lumen using sterile micropipette. All specimens then incubated for 48 hours at 37 ˚C.Intracanal sample collection by paper points showed an insignificant difference between different irrigation solutions tested and positive control Group at p=0.001. Group 1 showed a median of 8.9*104(CFU/ml),where group 2, 3, 4 and positive control groups showed mean values of 2.2*104, 7.3*104,8*104 and 3*104 (CFU/ml) respectively. Negative control group showed the highest significant values for colony forming units (118.4*104CFU/ml).