Introduction: Viral hepatitis C is one of the major health problems worldwide. Combining Interferon alpha with ribavirin in treatment of HCV proved to be the best regimen of treatment, yet it has a considerable percent of non-response. Aim: Evaluation of the relation between serum ferritin levels and erythrocytic reduced glutathione concentration, in chronic hepatitis C patients treated with combination therapy, and response to treatment. Methods: The study included 40 patients (20 responders to combination therapy, and 20 non-responders to the same regimen) and another 20 healthy subjects as controls for the comparison between serum ferritin and erythrocytic reduced glutathione with the other 2 groups. Results: On comparison of levels of erythrocytic reduced glutathione between responders (Range: 6.3-8.9M/gHb) and non-responders (Range: 3.3-4.5M/gHb) that it was statistically highly significant (P<0.00), but levels in both groups are still lower than the control group (Range: 10-15M/gHb). As regards the comparison of serum ferritin levels between responders (Range: 25-200ng/ml) and non-responders (Range: 98-643ng/ml), this was statistically highly significant (P<0.00). Conclusion: Non-response to combination therapy was associated with increased levels of serum ferritin and decreased levels of erythrocytic reduced glutathione.