Objectives: The aim of the present study is to evaluate the effect of an experimental bioactive glass-based nanopowder formula (BG-based formula) compared to Casein Phosphopeptide-Amorphous Calcium Phosphate Fluoride (CPP-ACPF) on remineralization and color change of experimentally induced white spot lesions (WSL) in enamel. Methods: The experimental nanopowder components {Calcium sodium phosphosilicate bioactive glass (BG), carbonated hydroxyapatite (CHAP) and fluorohydroxyapatite (FHAP)} had undergone characterization using XRD, SEM and EDXA. Twenty seven upper human permanent central incisors were collected, polished and randomly distributed into three groups (I, II and III). WSL were induced in all teeth. Each group was daily subjected to the remineralizing agents (14 days); group I (negative control) was suspended in artificial saliva, group II was treated with CPP-ACPF, while group III was treated with the slurry of the experimental BG-based formula. Each group was measured for color parameters L*, a* and b* and color difference (ΔE), at the baseline (M1), after formation of the WSL (M2), and after remineralization (M3). The microstructure and minerals content of enamel were examined for each group using SEM and EDXA at M1, M2, and M3. Results: Group III (the experimental BG-based formula) showed more regular deposition of the mineral layer on the surface. A subsurface EDXA profile of a sectioned representative tooth from each group showed that the experimental BG-based formula was effective in surface as well as subsurface remineralization of WSLs. Whereas, MI GC tooth paste plus showed remineralization of the enamel subsurface more than the enamel surface. Group II showed the least value of color difference between the remineralized lesions and the baseline, ΔE. This was followed by group III (BG-based formula), and group I (artificial saliva) respectively. Significance: Both CPP-ACPF and the new experimental BG-based nanopowder formula were effective in remineralization of enamel WSLs, with less surface remineralization in case of CPP-ACPF. The CPP-ACPF showed better color improvement than the experimental BG-based formula and the artificial saliva respectively.