Aim: The aim of this study was to evaluate the sealing capacity, bond strength and root fracture resistance of three different types of root canal sealing materials.Methodology: A total of 35 single-rooted human mandibular premolar teeth with single canals were instrumented using step back technique and divided into three groups; 10 each, and 5 as a control group. 30 of the prepared canals were obturated with lateral compaction technique using gutta percha and three different types of sealers; 10 with ZnOE based sealer, 10 with resin based sealer and 10 with silicone based sealer. Each root was sectioned horizontally into 3 portions; 2 mm slice for sealing capacity test, 2mm slice for bond strength test and 11mm for root fracture resistance test. A fluid filtration device was used to measure fluid penetration through the root canals. The adjusted hydrostatic pressure was applied to the apical side of the root disc, the fluid flowed out from the coronal side of the root disc. Permeability was measured by tracing the displacement of an air bubble on top of a millimeter scale for 24 hours. Bond strength test was done using the universal testing machine. Failure was manifested by extrusion of filling material from the canal and the maximum failure load was recorded. A random disc from each group was kept for qualitative evaluation of dentin\sealer interfaces with the SEM. Regarding root fracture resistance test, using the universal testing machine, failure manifested by an audible crack and load fracture value was recorded in Newton. The results were subjected to statistical analysis using Kruskal Wallis and Mann Whitney tests.Results: The sealing capacity and root fracture resistance of the silicone based sealer was the highest one, followed by the resin based sealer, while the ZnOE based sealer had the least mean values. While for bond strength test, the resin based sealer had the highest mean values followed by silicone based sealer and the least values were for the ZnOE based sealer.Conclusions: The compositional pattern of the tested root canal sealing materials affects dramatically their physico-mechanical behavior. Improved bonding of sealers to dentin does not necessarily mean the improvement of sealing ability, neither their fracture resistance.