Objectives: To fabricate and characterize chitosan/bioglass composite scaffolds with the evaluation of the effect of different compositional ratios on the biological and mechanical properties of the prepared scaffolds. Materials and methods: Scaffolds preparation: Four groups of chitosan(C) based scaffolds were fabricated using freeze drying technique; I1, I2, I3 and I4, with bioglass (B) percentages zero, %52, 502 and %52, respectively. The scaffolds were characterized using fourier transformer infrared spectroscopy (FTIR), scanning electron microscope (SEM), Mercury intrusion porosimeter (MIP), degradation test and mechanical testing. In addition, bioactivity of the prepared scaffolds was examined using stem cells via cell adhesion and osteogenic evaluation. Osteogenic evaluation of the scaffolds was performed using alkaline phosphatase activity assay and PCR test for osteonectin gene detection. Results: FTIR analysis showed shifting and deformation of some peaks indicating the formation of a composite scaffold. SEM images revealed an interconnected porous architecture, while MIP results showed that group I4 exhibited the lowest porosity percentage mean and group I1 exhibited the highest mean. Degradation results showed a significant decrease in degradation rate with increasing bioglass content, where group I4 showed a significantly lower degradation rate at the end of all time intervals. As regards to mechanical testing results, group I4 revealed the highest compressive strength and elastic modulus means while group I1 showed the lowest means. In addition, group I4 showed the highest bioactivity regarding cell adhesion evaluation and the performed osteogenic activity tests. Conclusion: This study suggests that increasing bioglass content up to %52 of the composite scaffold will yield a porous scaffold with satisfactory biological and mechanical properties to be used in tissue engineering.