Glucagon like peptide-1(GLP-1) is an incretin hormone which is responsible for insulin secretion in response to hyperglycemia .GLP-1 is secreted from intestinal cells .Both high fat diet ,high fructose intake contribute to development of insulin resistance .Rats used in the present study included normal control rats, high fat fed rats (40% fat) while the 3rd group received high fructose concentration in drinking water (60% fructose).Fasting blood samples were collected for the study of different parameters in fasting plasma, also post-prandial plasma for the study of GLP-1 level, systolic blood pressure was measured in the 3 groups. Results: The results of the present study was the development of insulin resistance with high fat diet, high fructose intake. In both insulin resistant groups, there was significant elevation of fasting plasma glucose, fasting plasma insulin, fasting plasma (cholesterol, triglycerides and LDL levels). On the contrary, there was highly significant reduction of post-prandial GLP-1 and fasting plasma HDL levels in comparison with control group. There was also rise of systolic blood pressure in insulin resistant rats. It is concluded that consumption of excess fat, high fructose intake in diet, play a role in increasing incidence of insulin resistance. Also the important finding of the reduction of post-prandial plasma GLP-1 level in insulin resistant rats is suggestive for the use of GLP-1 agonists or dipeptidyl-peptidase-IV (DPP IV) inhibitors as a line for treatment of type 2 diabetes mellitus.