Back ground: The promise of testosterone therapy may seem enticing, but there are lots of misconceptions about what the treatment can and can't do. Recently, much has been written about testosterone and male hormone replacement therapies. Many physicians are beginning to offer “age management” therapies to a wider range testosterone replacement therapies have become increasingly popular and of patients. Objective: The aim of the present study was to evaluate the effect of varies levels of testosterone on different metabolic parameters in male albino rats, as well as Real-time PCR GLUT 4 Gene expression level in adipose tissue and the size of visceral &subcutaneous fat cells. Design and method: The study was conducted on 40 male albino rats that were subdivided into four groups: group I which is the control group I, group II included castrated animals, group III included castrated animals that received 0,2 mg testosterone for 21 day and group IV included castrated animals that received 0.1mg testosterone daily for 21 day which is the duration of study. Result: The result of the present study revealed that at both level testosterone replacement therapy, produce significant decrease in serum glucose, insulin, cholesterol, triglyceride and LDL level & fat cell size & count in both visceral and subcutaneous adipose tissue when compared to castrated group and significant increase in serum HDL and total testosterone level when compared to castrated group. Measurement of the real time PCR GLUT 4 Gene expression level in visceral and subcutaneous fat cells of different study groups showed that there are significant increases in castrated groups receiving testosterone when compared to castrated group.