Nitrofurazone {NF;2-[(5-nitro-2-furanyl)-methylene] hydrazide carboxamide}, is a synthetic furan derivative, active against a broad spectrum of bacteria, which has been used both therapeutically and prophylactically against gastro-enteritis in a number of food-producing species, including sheep, goats, cattle, chickens, pigs, fish and turkeys. In non food-producing animals, pet animals, it is used in the treatment of wound and urinary tract infections. In human, it is used topically in the treatment of skin infections and burns and also as NF- impregnated urinary catheters to reduce the urinary tract infections in catheterized patients. This work was designed to study the histological and immunohistochemical changes that might occur in the testis of albino rat in response to a single oral administration of Nitrofurazone. This study was conducted on twenty-four male albino rats divided into two main groups; the experimental group (eighteen rats) which was given a single oral dose of 300 mg/kg Nitrofurazone, then subdivided into three subgroups A, B and C (six rats each), and the control group (6 rats). These three subgroups (A, B, and C) were sacrificed together with the rats of the control group (two rats with each subgroup), respectively after, two days, four days and four weeks from Nitrofurazone administration and the testes were excised. Haematoxylin (H.) and Eosin (E.) stained sections, immunohistochemical stained sections for FADD (Fas Associated Death Domain, which is an apoptosis marker) and semi-thin sections were prepared, followed by morphometric studies to assess the area percent of FADD positive nuclei and to count the number of Leydig cells in the interstitial tissue. Two and four days following Nitrofurazone administration, the spermatocytes and the spermatids in the H. and E. stained sections and in semi-thin sections showed features of apoptosis. They also showed positive immunoreactivity for FADD in immunohistochemical stained sections. Sertoli cells were affected in the form of vacuolation of the cytoplasm and enlargement of their nuclei. While, Leydig cells were histologically normal with increase in their count and spermatogonia were preserved.By four weeks following Nitrofurazone administration recovery was evident in the cells lining the seminiferous tubules. It could be concluded that single oral dose of Nitrofurazone induces histological and pathological changes in the testes of the albino rats that might cause male infertility.