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Efficacy of ELISA in diagnosis of schistosomiasis and monitoring of morbidity

Thesis

Last updated: 06 Feb 2023

Subjects

-

Tags

Parasitology

Advisors

El-Mattarawi, Ulfat M., Khattab, Hala M., Farrag, Nahed F.

Authors

Taher, Eiman El-Shabrawi

Accessioned

2017-03-30 06:23:50

Available

2017-03-30 06:23:50

type

M.D. Thesis

Abstract

This study aims to evaluate the sensitivity of two ELISA techniques versus ultrasonography in diagnosis of chronic hepatosplenic schistosomiasis and assessment of different degrees of morbidity,100 patients enrolled in this study were divided into 5 main groups : Group I with simple intestinal schistosomiasis, group II with periportal flbrosis with or without organomegaly, group III with hepatosplenomegaly with portal hypertension and group IV with advanced morbidity and end stage liver disease. Group V included 40 individuals as control group. All patients were subjected to stool examination, ultrasonographic evaluation and serological assessment using two techniques of ELISA conventional versus Dot-ELJSA with two types of antigens crude adult worm and soluble egg antigens assessing both IgG and IgM antibodies. Ultrasonographic evaluation proved to have the main role with the advance of the degree of morbidity and there was no relation between different stages of the disease and the degree of antibody positivity. IgM antibodies were more prevalent in the earlier stages of the disease while IgG antibodies were more consistent in different stages of the disease. Based on the conditions of antigen preparations used conventional ELISA was more sensitive compared to dot ELISA with the adult worm antigen was more sensitive than the soluble egg antigen.

Issued

1 Jan 2006

Details

Type

Thesis

Created At

28 Jan 2023