This work presents a comparative study between the flexural behavior of traditionally cast largescale reinforced concrete (RC) T-beam made of natural aggregate concrete (NAC) and the response of a similar semi-precast RC beam made of both NAC and recycled aggregate concrete (RAC). The semi-precast beam was composed of a precast U part, in which both the beam longitudinal tensile reinforcement and the transverse reinforcement were built-in. The core of the beam web and the beam flange were cast using RAC. Both beams were simply supported and tested under the effect of two-point loading system. The shear span to depth ratio of both beams was equal to 3.0. The test results pointed to the successful production of a new generation of semi-precast RC beams using both NAC and RAC that can ensure the required durability and achieve a comparable flexural response to that of the traditional RC beam.