Fiber reinforced polymers (FRP) is widely used in strengthening and repairing of reinforced concrete structures. This is due to the benefits of FRP materials against commonly used systems especially high strength and durability. One of the new advances in the field of FRP is using of externally bonded prestressed FRP laminates in strengthening of flexure members which combines both the advantages of FRP and prestressing by improving serviceability, increasing of stiffness and increasing load carrying capacity. This paper is a part of a study that experimentally and numerically, investigates the behavior of the flexural Reinforced Concrete (RC) slabs with prestressed Carbon Fiber Reinforced Polymer (CFRP) laminates. Different anchoring methods for CFRP laminates were investigated, also, a new prestressing system was developed to be able to prestress the CFRP laminates. Results of different anchoring methods tested under tension force are presented. In addition, two trials for prestressing systems invistigated to find the best anchoring and prestressing method. The prestressing system was tested in strengthening eight full scale simply supported reinforced concrete slabs of 4000mm free span, 500mm width and 200mm thickness, tested under 4 point flexural load. Results of four slabs are presented in this paper to evaluate the efficiency of the anchoring and prestressing system.