Structural members in the form of deep beams are present in many applications. The presence of openings in the critical shear zones may affect the ultimate capacity or the serviceability requirements. The current codes of practice give simplified design methods for deep beams without special consideration for the effect of openings. Also, the available literature is insufficient in all possible cases for predicting the behaviour of such deep beam with opening. An experimental program includes ten reinforced concrete deep beams were tested under concentrated load, such that to study the behavior of deep beam with strut and tie model theory. The beams were simply supported on rigid steel plates of 800 mm apart (span length) and with overhanging 150 mm from each side, the total height of the beam and its thickness are 800 mm and 100 mm respectively. Shape, size and location of opening are considered in this study. The first beam is control beam without openings, three beams with square openings of 120 mm x 120 mm, with different locations, another three beams with rectangular openings 160 mm x 80 mm, with length in vertical direction, while the last three beams have rectangular openings 160 mm x 80 mm, with length in horizontal direction. Results indicated that all deep beam with opening have failure load less than its control beam. It also indicated that either moving of opening far from the imagine width path of forming strut member from concentrated load to each support of the deep beam or decreasing the opening size, the failure load decreases , bottom deflection decreases and corresponding ductility increases.