The present paper demonstrates the findings related to eccentricity shift of external prestressing of segmental girders as a part of a comprehensive research. The investigation included an experimental program along with an analytical study to interpret the monitored observations of precast prestressed segmental beams with different detailings. All specimens of the case studies were T-sections with end rectangular block to allow fixtures of the two external straight 7-wire strands above the bottom soffit of the girders with and without aligning deviators. For comparison purposes, an externally prestressed monolithic beam was considered. On the other hand, shear keys were fabricated for the joints of the segmental beams. Except for a specimen with internal unbonded end anchored tie rods and another specimen with embedded shear friction dowel between segments, plain dry joints were considered in other cases. Under monotonic four-point loading, cracking pattern, failure mechanism, load-deflection relationship, incremental prestressing strain, interfacial segments separation and moment-curvature characteristics were the salient features discussed in the overview. Considerable stiffness degradation was remarkable for all segmental beams from the early stages due to loss of overall integrity. Existence of deviators in segmental beams tended to slightly maintain higher overall stiffness relative to those with free strands followed by specimen with internal unbonded end anchored tie rods then by that of joints with shear friction dowels which provided the highest integrity closest to the monolithic specimen.